WinCAM NDVI Analysis System
Image Analysis for Plant Science
Regent Instruments Inc. since 1991




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WinCAM, NDVI, color analysis, measurement, CAM, color area meter  
WinCAM NDVI Measurements
1° Color Morphology Analysis

In the first phase of the analysis, the image colors are analysed and quantified. It is used to differentiate objects from their background. Debris can be filtered out by different methods (see table below). In the second analysis phase, measurements are made on the detected objects and the whole image (or analysed region of it). These fall into three categories.

  • 1) Global measurements related to the analysed region such as total and percentage area occupied by objects and their average morphological measurements (area, length, width...). Color aggregation is also in this group.
  • 2) Individual morphological measurements such as area, length, width, perimeter and form coefficient.
  • 3) Area in function of color, NDVI or Color Index. For each object and the analysed region, you get the area occupied by colors of interest and groups of them. This also includes information about the dominant color. Objects can also be classified based on color content in function of criteria that you choose.
The histogram above the image can display the area per color class or group and the objects’ frequency distribution in function of a morphological measurement (Ex: number of objects of a certain area or length range).
WinCAM, NDVI, color analysis, measurement, CAM, color area meter
WinCAM, NDVI, color analysis, measurement, CAM, color area meter
WinCAM, NDVI, color analysis, measurement, CAM, color area meter
WinCAM, NDVI, color analysis, measurement, CAM, color area meter

A 24 bits color image to be analysed. It can contain millions of colors. Such images are easily obtained with digital cameras and scanners.

Before analysing images, you must teach WinCAM which colors are part of the objects (grass, fruits, disease or healthy regions) and which ones are the background (soil) simply by clicking them in the image and giving names to these clusters that we call “color classes”. In the first phase of the analysis, WinCAM classifies the image pixels into one of these classes. The result of this classification can be displayed and saved to files. Color classes can be grouped. The group can be given a name and area measurements data can be obtained per color class or group. In the above example, the Grass group is made of four green colors and the soil two brown colors.
The classification into groups can also be displayed and saved to files. There can be up to three groups and each one can have up to twelve color classes.
Example of color classes to analyse the soil cover image to the left.
Pixels are classified as a function of their color (Hue, Saturation and Intensity which is equivalent to Hue, Value and Chroma used in the Munsell color classification system). A tolerance factor for each of these component allow to include more or less colors in each class.
WinCAM, NDVI, color analysis, measurement, CAM, color area meter WinCAM, NDVI, color analysis, measurement, CAM, color area meter WinCAM, NDVI, color analysis, measurement, CAM, color area meter
WinCAM NDVI can be used to quantify toasts (slices of bread) baking uniformity.
The image of colour classes. It shows into which color class each pixel has been classified.
Colour classes and groups defined
to analyse the toasts baking.

Note: You can get interactive information about analysed objects by clicking them in the image or look at the data files for more details. Measurements data are saved in text files that can be read by many programs (like Excel).

When you classify soils or plants by comparing their color to a printed set of reference colors such as the Munsell Soil Color Charts or Munsell Plant Tissue Color Chart, you are doing what we call an absolute classification. When you classify plant colors without comparison to an external reference, simply by comparing them you are doing what we call a relative color analysis. WinCAM NDVI works in the latter way. It classifies colors on a relative basis of what is contained in an image. It is possible in WinCAM NDVI to emulate an absolute color analysis by including in each image some reference colors (such as a subset of the Munsell samples). In that case, colors of the objects to analyse are compared to these reference color samples. This will work for as long as the number of reference color samples does not exceed WinCAM NDVI's limit of 36.

2° Spectral Vegetation Index Analysis (NDVI, Greenness)

WinCAM NDVI can compute six vegetation indices frequently used in agriculture and forestry healthiness/stress assessment (NDVI, ENDVI, EVI2, MSAVI, WDRVI, Green Chlorophyll) and you can also define you owns.

- The NDVI (Normalized Difference Vegetation Index) is one of those pre-defined spectral indices. It is a measure of plant healthiness and quantity. It is computed from images which have NIR (Near InfraRed) data in one of its channel and Visible data (blue or red) in another. Such images can come from aerial or ground based multi-spectral cameras. The index highlight the strong reflectance of healthy green plants in the NIR (due to mesophyl cells scattering light at that wavelength) compared to the low reflectance in the Visible (due to the chlorophyll high absorption). Dry or inactive vegetation, soil, rocks and water do not exhibit this spectral behavior. NDVI images and data can be analysed interactively or in batch (without operator supervision) from multispectral images stored in a folder.

WinCAM, NDVI, color analysis, measurement, CAM, color area meter
WinCAM, NDVI, color analysis, measurement, CAM, color area meter
This image was acquired with a conventional color camera
This image was acquired with a NDVI camera. Its Red channel contains the NIR data while the Blue one contains the visible Blue channel data.
This image displays the NDVI data using a gray scale (white = high NDVI value, Black = negative values). It was produced in WinCAM from the above row NDVI camera image.
This image (and the two below) displays the NDVI data using 3 different (false) color schemes. It was produced in WinCAM from the left NDVI camera image.
These images display the NDVI data using 3 different (false) color schemes. It was produced in WinCAM from the above NDVI camera image.

- You can also can define your own Spectral (color) index as a mix of an image RGB (Red, Green or Blue) or HIS (Hue, Saturation, Intensity) channels or the ratio of two such mixes. The Greenness is an example of a custom spectral index you can define. It quantifies the amount of green in a standard color image. It is an index which has positive values when green is present and zero or negative when it is not. It is frequently used in soil vegetation cover analysis. Spectral (color) indices images and data can also be analysed interactively or in batch (without operator supervision).

WinCAM NDVI can compute the spectral indices for the whole image, a region of it or per objects. It can also use the index values to classify them on different accept/reject criteria. Index data are available for these 3 types of analysis on screen and in data files (text based) and is also available in visual form as an index image with gray levels representing the index strength. These images can be saved for analysis or imported in different programs for multiple purposes (generate reports,...).

3° Profilometry

WinCAM NDVI can be used as a profilometer to analyse the gray levels (light intensities) or color variations along paths drawn by the operator over the image. Gray levels profiles can be saved in data files while color profiles’ data are saved as a summary of their color content (length in units and percentage of total path length per color). The profilometer can also be used to manually or semi-automatically measure objects dimensions by clicking them in the image.

4° Reflectance and Color Balance Calibration

It is possible to calibrate WinCAM NDVI so that it makes measurements in reflectance values or simply to balance the color channels. The former is done using known reflectance multi steps targets and the latter with a single white object. Both are available for the Visible and NIR spectrum. Calibration against know standards allows you to compare analysis data between images acquired with different cameras or camera settings (thus different lighting intensities for the same reflectance).

5° Its ability to analyse individual objects

WinCAM NDVI has the ability to analyse individual objects such as rice kernels (to measure their morphology, classify them in function of color or quantify the colors distribution per kernel).

The analysis of soil covered by plant,s as illustrated at 1° in the introduction. There are no objects to analyse in such images (only the classification of pixels into groups such as healthy vegetation, diseased vegetation and soil).

WinCAM, NDVI, color analysis, measurement, CAM, color area meter
WinCAM NDVI Pro detailed features & measurements
Analyse whole image or sub-region of it Analyse whole image or a sub-regions of it of any shape. Rectangular and circular are pre-defined shapes or create irregular shape regions with the lasso tool (outline their boundary by drawing in the image).
Individual objects analysis Analyse non-touching objects morphology and color on an individual basis. Length can be measured by 4 methods, width, perimeter, form coefficient, length/width ratio, object area, area per color and information about dominant color. Objects can be classified in function of different criteria (color, index….).
6 Pre-defined Spectral Indices NDVI (Normalized Difference Vegetation Index), ENDVI, EVI2, MSAVI, WDRVI, Green Chlorophyll.
User definable Spectral Indices You can define your own color indexes by combining the camera channels.
Profilometer To record and analyse light intensities or color variations along paths drawn by the operator over the image.
Debris filtering based on morphology Remove debris based on anatomical properties like area, length, width, length/width ratio and form coefficient.
Debris filtering based on color When debris have a different color than the objects to analyse, this function can be used to exclude them from the analysis
View/Analyse/Export RGB or HIS channels From a color image, choose its Red, Green, Blue or Hue, Saturation or Intensity component to be displayed on screen, analysed and optionally saved to file. Note: The HSI components are equivalent to Munsell's Hue, Value and Chroma components.
Exclusion regions Exclude from the analysis any sub-region(s) of any shape of the image. Can be used to remove debris or to avoid areas which must not be analysed.
Batch analysis Analyse images without operator supervision. Note: Images which require operator intervention for calibration cannot be analysed in batch.
Pixel size calibration For images from camera, pixels size must be determined by calibration to produce measurements in units of your choice (mm, cm, inches...). With Regent's targets (see PICK), all is needed is a mouse click on the target in the image..
White balance calibration To remove color variations due to changes in lighting or camera settings between images. With Regent's targets (see PICK), all is needed is a mouse click on the target in the image.
Multi steps reflectance calibration Reflectance calibration can be done using multi-steps targets (not included) to get reflectance measurements data and image.
Remove background light variations For images acquired with a camera when background light has variations which can affects the analysis. Note: It is best to invest time in having a uniform lighting. The analysis will be faster and easier.
Image edition To remove debris or artefacts. Can also be used to make corrections to pixels classification (into object and background), to draw over objects to analyse (when this is not possible automatically).
Interactive measurements/Number of methods Interactive measurement is a way of measuring objects manually (both versions) or semi-automatically when the automatic analysis cannot be used. In semi-automatic mode, simply draw a line over an object at the place you want it to be measured and WinCAM NDVI automatically find the object's boundary beginning and ending over that line and measure the length in between (it will also measure the traveled distance).
Histogram of frequency distribution Display the number of objects that fall into classes of morphological features (Ex: number of objects with an area between 1 and 2 square mm).
Histogram of color distribution Display the area covered by each color class or group in the analysed region or image.
Extract GPS data Extract GPS data (Latitude, Longitude, Altitude, Direction, Date, Time) when they are present in tiff or jpeg image files. These data are saved with the measurements data.
Display image data under mouse cursor Displays the pixel value at the mouse cursor position in the image. The data written depends on the image displayed. It can be; the RGB, Gray levels, Hue, Saturation, Intensity or Spectral index/NDVI value.