WinFOLIA is for Leaf Analysis
Image Analysis for Plant Science
Regent Instruments Inc. since 1991




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Systems Specifications

WinFOLIA is available as software alone or as complete system

Model Selection and Image Transfer to WinFOLIA

WinFOLIA uses the TWAIN1 protocol to communicate with scanners and get images directly from them (without saving them to disk) in a very fast process. There are many versions of TWAIN and some features are not mandatory for a scanner to be TWAIN compliant. Regent Instruments cannot test all scanners and drivers available on the market so we do not guarantee that they will be compatible with all features of WinFOLIA. We have tested and selected a few models that are representative of their categories and we offer them for sale with WinFOLIA as complete systems (WinFOLIA can also be purchased as software alone). These models meet the requirements for precise scientific work and we give them a speed rating to help you choose one. There are important differences in terms of image quality and speed among different models. We retain those that are robust, reliable, will last long and which come with good warranty support from their manufacturer. With all scanner models it is possible to scan leaves using the scanner manufacturer program, save the image to disk and then open and analyse those images in WinFOLIA2. This process is slower than TWAIN because it involves two extra steps.

We calibrate the scanners we sell for precise measurements. Scanners are mainly made for artistic applications. When you set it to scan at 400 dpi, it might in reality scan at 392 or 410 dpi. This is what our calibration compensates for. Our calibration is permanent and is automatically used by our programs to compensate for differences among scanners so that data produced by different models or different units of the same model will give similar results. Our calibration also compensates for differences when using a single scanner under different conditions such as when measuring an object at various orientation on its scan area and, for dual lenses scanners, when using different lenses.

Our scanners come with additional instruction manuals that explain how to install the scanner with our accessories and how to scan biological samples for analysis with our programs. These help you to get the best images from it and to save time by giving some tips that are specific to the scanner that you have purchased (optimizing it for speed vs. accuracy or avoiding frequent beginners errors). We know the scanners we sell for using them in the same conditions as you with WinFOLIA so we can easily help in case of problems. Because testing scanners is a continuous non-neglectable time and money investment, we do not disclose the scanner models we sell, neither do we give our opinion on specific models. If you prefer to buy a scanner locally, we suggest to test it before purchase and to select a reseller that will be able to assist you after as Regent Instruments will not provide technical assistance for scanners not purchased from us.

Our scanners also come with their manufacturer’s software program(s) which allow to use them for other generic applications (object, photo, slide or document scanning).

1 TWAIN drivers are installed in your Windows operating system when you install the scanner manufacturer software programs.
2 Files must be in tiff uncompressed grey levels (8 bits/256 grey levels) or colors (24 bits/millions of colors) or jpeg format.

Although WinFOLIA can obtain and analyse images directly from many scanners, it is advantageous to purchase one from Regent for the reasons given here.


Digital Cameras
Model Selection and Image Transfer to

Some reasons for using cameras instead of scanners are that they can take images of larger objects (albeit at lower resolution), are (most of the time) more portable or simply because you might already have some at hand (they are quite cheap and common these days). WinFOLIA can open and analyse jpeg images files produced by almost any digital camera (RAW files do not work). The fastest way to measure leaf area and morphology in the field is when the computer can get instantaneous image transfer from the camera to it. This is possible if your camera has a TWAIN driver (this is rare) or that it can be remotely controlled from the computer (with the camera manufacturer software) and transfer images directly to it via a wire (such as USB2) or a wireless (WiFi) connection. In those cases, WinFOLIA can analyse images as soon as they are saved to disk (or directly from the camera if a TWAIN driver is available). It is also possible to transfer one or more images at a time using the following methods:

  1. Remove the memory card from the camera and insert it in a card reader connected to the computer (some computers have built-in card readers). The memory card appears as a removable disk on Windows computers. The image files they contain can be opened from WinFOLIA or they can be transferred from the card to the computer hard disk prior to this.
  2. Many digital cameras can be mounted as a removable disk simply by connecting them to the computer (after installation of their manufacturer drivers). WinFOLIA can access these images directly or you can transfer them to the computer and open them from there.
  3. You can use the software program that came with the camera to transfer images in lot and analyse them later in WinFOLIA.
WinFOLIA can analyse images interactively (one after the other) or in batch (without operator supervision)1. This will work if you have the right calibration setup and no unwanted objects in the image (feet, debris, etc....). This allow to acquire images rapidly in the field and to analyse them later in the lab (with or without supervision).

1 Not all analyses can be done in batch (envelope and teeth for example).

Data produced by WinFOLIA are saved in standard ASCII text files. They can easily be opened by statistical or spreadsheet programs such as Microsoft Excel. The analysis settings are saved with the measurements data (useful to make verifications or quality control on the measurements). Furthermore, when an image is saved to a tiff file after it has been analysed, the analysis is also saved in it and when such file is later opened from WinFOLIA, the analysis is recreated as it was at saving time.

WinFOLIA comes with printed color manuals which are abundantly illustrated. Like all of Regent Instruments’ products, it is backed with prompt and competent technical support by email (messages are typically answered within one hour when they are received on working hours Eastern time America). WinFOLIA is updated regularly (typically once a year) to add new features or simply to keep it on par with new operating systems as they become available.

WinFOLIA has a companion program called WinSEEDLE for conifer needles and seeds analysis. Both programs can share the same image acquisition hardware (you must specify it at purchase time). A rebate is offered when both products are purchased together. WinFOLIA and WinSEEDLE are members of a family of products sold by Regent Instruments for plant science research and analysis. Among the other products you will find WinRHIZO for root analysis and WinDENDRO for tree ring analysis. See our web site for more information.

WinFOLIA software program
WinFOLIA is available in three different versions; Basic, Regular and Pro. For more informations regarding measurements and features offered by each version, click here.
Recommended computer configuration
Image analysis programs are extremely demanding on computer resources (more than a word processor for example), so it is a bad idea to use an old computer for this type of application. Most computers sold today have the minimal power. Although a minimum configuration can work, you will gain a lot in productivity by adding some little extras like a large screen (22"-24"), plenty of RAM (over 2GB). It works with Windows XP, Vista, Windows 7 (32 or 64-bit).
Buy more than one system or buy WinFOLIA with any other product made by Regent Instruments. When WinFOLIA is ordered with another software of Regent Instruments, a 10% discount is offered on the least expensive one.